Effect of pre-existing maternal obesity, gestational diabetes and adipokines on the expression of genes involved in lipid metabolism in adipose tissue

M Lappas

Journal article

Effect of pre-existing maternal obesity, gestational diabetes and adipokines on the expression of genes involved in lipid metabolism in adipose tissue

M Lappas

Metabolism Clinical and Experimental | W B SAUNDERS CO-ELSEVIER INC | Published : 2014

DOI: 10.1016/j.metabol.2013.10.001

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Abstract

Objective To determine the effect of maternal obesity, gestational diabetes mellitus (GDM) and adipokines on the expression of genes involved in fatty acid uptake, transport, synthesis and metabolism. Materials/Methods Human subcutaneous and omental adipose tissues were obtained from lean, overweight and obese normal glucose tolerant (NGT) women and women with GDM. Quantitative RT-PCR (qRT-PCR) was performed to determine the level of expression. Adipose tissue explants were performed to determine the effect of the adipokines TNFα, IL-1β and leptin on adipose tissue gene expression. Results Pre-existing maternal obesity and GDM are associated with decreased expression in genes involved in fat..

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University of Melbourne Researchers

Martha Lappas Author

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Grants

Awarded by Diabetes Australia Research Trust

Funding Acknowledgements

Dr Martha Lappas was a recipient of a National Health and Medical Research Council RD Wright Fellowship (grant no. 1047025). The work described in this manuscript was funded by the National Health and Medical Research Council (grant no. 454310), Diabetes Australia Research Trust (DART) and Medical Research Foundation for Women and Babies.